Post by Max on Jun 30, 2005 7:31:22 GMT -5
Interaction with human Oxytocin gene promoter
A retinoic acid response element (RARE) was identified in the human oxytocin (OT) gene promoter by DNA-mediated gene transfer techniques. RA elicited a marked stimulation of the transcriptional activity of the OT promoter in cells cotransfected with either the human RA receptor alpha, beta, or gamma. In cells cotransfected with the RA receptor alpha, the ED50 of this response was 5 x 10(-10) M. The RA response could also be conferred to a heterologous promoter independent of orientation. 5'-Deletions as well as site-directed mutations demonstrated that four TGACC motifs, located at -162, -156, -103, and -83 in the OT promoter, are necessary for optimal RA induction. Mutation or deletion of any of these elements reduces significantly the RA response. The first two TGACC motifs overlap with the estrogen response element that we have previously characterized in this gene. Furthermore, the TGACC motif located at -83 overlaps with the CCAAT box [1]. A significant suppression of the oxytocin gene in doses associated with exposure in human acne subjects can not be excluded.
GnRh
FSH/LH
A retinoic acid response element (RARE) was identified in the human oxytocin (OT) gene promoter by DNA-mediated gene transfer techniques. RA elicited a marked stimulation of the transcriptional activity of the OT promoter in cells cotransfected with either the human RA receptor alpha, beta, or gamma. In cells cotransfected with the RA receptor alpha, the ED50 of this response was 5 x 10(-10) M. The RA response could also be conferred to a heterologous promoter independent of orientation. 5'-Deletions as well as site-directed mutations demonstrated that four TGACC motifs, located at -162, -156, -103, and -83 in the OT promoter, are necessary for optimal RA induction. Mutation or deletion of any of these elements reduces significantly the RA response. The first two TGACC motifs overlap with the estrogen response element that we have previously characterized in this gene. Furthermore, the TGACC motif located at -83 overlaps with the CCAAT box [1]. A significant suppression of the oxytocin gene in doses associated with exposure in human acne subjects can not be excluded.
GnRh
FSH/LH
