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Post by Max on Jul 12, 2005 17:29:35 GMT -5
Signifícant upregulation of thrombomodulin during (Ro)accutane exposure, unknown effects post exposure
Thrombomodulin, a major anticoagulant proteoglycan of the endothelial cell membrane, is a thrombin receptor that acts as a cofactor for protein C activation. Retinoic acid induced significant increases in the total antigen level and in surface and intracellular thrombomodulin activities only in keratinocytes grown in a low-calcium medium. In these undifferentiated keratinocytes, quantification of mRNA transcripts showed a threefold increase after retinoic acid stimulation [1].
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Post by Max on Jul 12, 2005 17:29:58 GMT -5
b
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Post by Max on Jul 12, 2005 17:30:22 GMT -5
References:
[1] Senet P, Peyri N, Berard M, Dubertret L, Boffa MC.
Thrombomodulin, a functional surface protein on human keratinocytes, is regulated by retinoic acid.
(1997) Arch Dermatol Res. Feb;289(3):151-7.
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Post by Max on Jul 12, 2005 17:34:43 GMT -5
Blood. 2001 Feb 15;97(4):946-51. Related Articles, Links
Regulation of human coagulation factor X gene expression by GATA-4 and the Sp family of transcription factors.
Hung HL, Pollak ES, Kudaravalli RD, Arruda V, Chu K, High KA.
Department of Pediatrics, University of Pennsylvania, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
Serine protease factor Xa plays a critical role in the coagulation cascade. Zymogen factor X is synthesized and modified in the liver. To understand the mechanisms governing the liver-specific expression of factor X, the proximal promoter of human factor X was previously characterized. Two crucial cis elements at -73 and -128 and their cognate binding proteins, HNF-4 and NF-Y, respectively, were identified. In this report, studies are extended to 3 additional cis elements within the factor X promoter. Using gel mobility shift assays, the liver-enriched protein GATA-4 was identified as the protein binding to the GATA element at -96. GATA-4 transactivates the factor X promoter 28-fold in transient transfection experiments. It was also determined that the Sp family of transcription factors binds 2 DNase I-footprinted sites at -165 and -195. Disruption of Sp protein binding at either site reduces the promoter activity by half. Simultaneous disruption of both sites reduces the promoter activity 8-fold. This is the first report indicating the involvement of GATA-4 in the regulation of clotting factor expression. These observations provide novel insight into mechanisms by which the vitamin K-dependent coagulation factors are regulated.
PMID: 11159521 [PubMed - indexed for MEDLINE]
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Post by Max on Jul 12, 2005 17:48:12 GMT -5
Biochemistry. 1991 Feb 12;30(6):1571-7. Related Articles, Links
Intron-exon organization of the human gene coding for the lipoprotein-associated coagulation inhibitor: the factor Xa dependent inhibitor of the extrinsic pathway of coagulation.
van der Logt CP, Reitsma PH, Bertina RM.
Haemostasis and Trombosis Research Unit, University Hospital, Leiden, The Netherlands.
Blood coagulation can be initiated when factor VII(a) binds to its cofactor tissue factor. This factor VIIa/tissue factor complex proteolytically activates factors IX and X, which eventually leads to the formation of a fibrin clot. Plasma contains a lipoprotein-associated coagulation inhibitor (LACI) which inhibits factor Xa directly and, in a Xa-dependent manner, also inhibits the factor VIIa/tissue factor complex. Here we report the cloning of the human LACI gene and the elucidation of its intron-exon organization. The LACI gene, which spans about 70 kb, consists of nine exons separated by eight introns. As has been found for other Kunitz-type protease inhibitors, the domain structure of human LACI is reflected in the intron-exon organization of the gene. The 5' terminus of the LACI mRNA has been determined by primer extension and S1 nuclease mapping. The putative promoter was examined and found to contain two consensus sequences for AP-1 binding and one for NF-1 binding, but no TATA consensus promoter element.
PMID: 1993173 [PubMed - indexed for MEDLINE]
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Post by dsfgh on Sept 7, 2018 19:53:13 GMT -5
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